RNA-seq: from reads to differentially expressed genes – applications close 7 Sep

Submit an application to join this hands on 2 day workshop in RNA-seq analysis.

About this workshop

RNA-seq is a popular method for simultaneously measuring and comparing the expression of tens of thousands of genes in multiple samples. Attendees can expect to gain an understanding of approaches to and practical experience in: quality control of short reads, alignment, gene counts, data normalisation, differential expression and visualisation. Places are limited to encourage discussion and experienced RNA-seq and Galaxy trainers will be present to answer your questions. The workshop materials are based on the following Galaxy training workshops:

• RNA-seq reads to counts
• RNA-seq counts to genes.

This training workshop will be conducted using Galaxy. It is important that you are familiar with navigating the Galaxy platform as the workshop will not include introductory content on how to use the platform. Attendance at a previous Galaxy workshop or working through this material is sufficient for attendance.

Lead trainers

Ms Jessica Chung & Dr Tom Harrop (Melbourne Bioinformatics)

Learning objectives

By the end of the workshop you should be able to:

• perform quality control of short reads using FastQC
• map reads to a reference genome using HISAT
• determine the number of reads mapped across genes or exons
• normalise read counts to account for composition bias
• perform differential expression analysis
• visualise results using a variety of plots.

Who is the workshop for?

This free workshop is available to staff and students at The University of Melbourne and its affiliated institutes only (refer to ‘Members’ section). It is aimed at researchers who have, or will soon acquire, RNA-seq short read data that they need to analyse themselves. Required: This is a hands-on workshop is being delivered over Zoom and using the Galax Australia open web based platform for analysis of data so attendees must have their own laptop with access to the internet/wifi and should register for a Galaxy account using their institutional email address. No additional software needs to be installed for this workshop and no progamming experience is necessary.

How to apply…

The workshop is free but due to anticipated high demand, participation is subject to application with selection. Places are strictly limited to 30 participants. Deadline for applications is 5pm, AEDT Tuesday 7 September 2021. Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application (successful, waiting list, unsuccessful) by 5pm, Friday 10 September 2021. If you require any further information, please contact us at bioinformatics-training@nullunimelb.edu.au.