RNA-seq analysis: from reads to differentially expressed genes

Overview

Lead trainers: Jessica Chung (Melbourne Bioinformatics) & Maria Doyle (Peter MacCallum Cancer Centre)

Target audience

Researchers who have, or will soon acquire, RNAseq short read data that they need to analyse themselves. Due to anticipated high demand and a limited number of places available at this workshop, places will be allocated based on an expression of interest application (opening midnight 30 October). Please complete the expression of interest form if you are interested in attending this workshop. Organisers will contact successful applicants by Wednesday 11^th of November.

Eligibility

This workshop is only open to staff or students of the University of Melbourne and its’ affiliated institutes (refer ‘Members’ section). Applicants will be required to enrol using their institutional email.

Pre-requisites

• some experience using the Galaxy platform
• expectation to perform RNAseq analyses as part of your research.

Workshop Outline

Across two days attendees can expect to gain an understanding of approaches to, and practical experience in, quality control of short reads, alignment, gene counts, data normalisation, differential expression and visualisation. Places are limited to encourage discussion. Our very experienced RNA-seq and Galaxy trainers will be present to answer all your questions. The workshop materials are based on the following Galaxy training workshops: https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/rna-seq-reads-to-counts/tutorial.html https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/rna-seq-counts-to-genes/tutorial.html