RNA-seq: from reads to differentially expressed genes – applications close 5 Feb
Submit an application to join an interactive workshop on RNA-seq analysis using Galaxy…
About this workshop
RNA-seq is a popular method for simultaneously measuring and comparing the expression of tens of thousands of genes in multiple samples.
There are many steps involved in analysing RNA-Seq data. The analysis begins with sequencing reads (FASTQ files). These are usually aligned to a reference genome. The number of reads mapped to each gene can then be counted. This results in a table of counts, which is what we perform statistical analyses on to determine differentially expressed genes and pathways.
This practical workshop provides a hands-on introduction to RNA-seq analysis from reads to counts, to differential expression. The workshop makes use of Galaxy, an online platform for biological research that allows people to use computational data analysis tools and workflows without the need for programming experience.
The workshop materials are based on the following Galaxy training workshops:
Ms Jessica Chung & Dr Tom Harrop (Melbourne Bioinformatics)
By the end of the workshop you should be able to:
- perform quality control of short reads using FastQC
- map reads to a reference genome using HISAT
- determine the number of reads mapped across genes or exons
- normalise read counts to account for composition bias
- perform differential expression analysis
- visualise results using a variety of plots.
Who is the workshop for?
Researchers who have, or will soon acquire, RNA-seq short read data that they need to analyse themselves. No prior experience with RNA-seq analysis is required.
The workshop requires you to be familiar with using and navigating Galaxy Australia.
If you are new to the Galaxy platform (an online platform for computational analysis) we recommend that you work through the Galaxy 101 For Everyone tutorial in advance of the workshop.
How to apply…
The workshop is free but due to anticipated high demand, participation is subject to application with selection. Places are strictly limited to 30 participants.
Ineligible or incomplete applications will not be considered. For your application to be considered you must:
- have an Australian institutional email address
- have, or will soon acquire, RNA-seq data
- complete the application form using registration link below.
Applications will be reviewed by the organising committee and all applicants will be informed of the status of their application (successful, waiting list, unsuccessful) by Tuesday 16 February 2021.
Deadline for applications is 5pm, AEDT Friday 5 February 2021.