QIIME2 analysis of bacterial 16s rRNA – applications close 2 August
Ashley Dungan, School of Biosciences, The University of Melbourne
Who is the workshop for?
Researchers and students at The University of Melbourne and its affiliated institutes only (refer to ‘Members’ section) who are new to analysis of bacterial communities using16sRNA who have some command line experience.
How to apply…
The workshop is free but due to anticipated high demand, participation is subject to selection through an expression of interest process.
Opening – 12noon, 20 July 2021
Deadline – 5pm, Monday 2 August 2021
Sumissions will be reviewed by our training team and all applicants advised of the status of their application (successful, waiting list, unsuccessful) by Thursday 5 August 2021.
This online, hands-on workshop will be conducted online using Zoom, Slack and a remote (cloud) computer. Attendees must supply their own charged laptop with access to the internet/wifi and with the following software preinstalled:
- Windows users: A terminal emulator such as PuTTY (free and open-source) will need to be downloaded.
- Software for file transfers between a local computer and remote server such as WinSCP or FileZilla.
- Mac users: No additional software needs to be installed for this workshop.
Knowledge/experience of the command line is a necessary for this workshop. It will be run on a Nectar Instance. You will be given an individual IP address and password to log on to using the SSH client tool on your own computer (Terminal on Mac or PuTTY on Windows) and navigate the directory structure and `scp` files from a remote computer to your local computer.
This workshop will give you an introduction to the QIIME2 analysis platform using 16S rRNA amplicon data from coral-associated bacteria. Topics covered include:
- importing, cleaning and quality control of the data
- taxonomic analysis
- building a phylogenetic tree
- basic visualisations and statistics.
By the end of the workshop you should be able to:
- take raw data from a sequencing facility and end with publication quality graphics and statistics
- answer the question “What is the influence of genotype (intrinsic) and environment (extrinsic) on anemone-associated bacterial communities?”
For further information, email firstname.lastname@example.org.